The lab suggests fixation will lead to the dyes to leak in the cells. I feel the assumption is the fact that fixation may perhaps harm cell membranes. I did find a paper which describes fixation employing methanol or acetic acid, just after staining by using a DCFDA spinoff. DCFDA is definitely the reagent that stains ROS from the package ab113851,
Your own info will be utilized to assist your working experience throughout this Web page, to handle usage of your account, and for other applications described within our privacy coverage.In our knowledge, running the stream cytometer with cells in media didn't appreciably impact the data – there was marginally fewer “background” Should the
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